Citation Details

UVA-induced cell cycle progression is mediated by a disintegrin and metalloprotease/epidermal growth factor receptor/AKT/Cyclin D1 pathways in keratinocytes

Authors	He YY, Council SE, Feng L, Chignell CF
In	Cancer Res (2008) 68(10): 3752-8
Genes of Interest	cyclin D1, ADAM17
Cells used in publication	HaCaT Epithelial Cells Cell Lines Species: human Tissue Origin: dermal
Substrate	siRNA
Application	RNAi
Topic	Signal transduction
Research Areas	Cancer Research/Cell Biology Dermatology/Tissue Engineering
Relevant Products	Nucleofector Device Cell Line Nucleofector Kit V
Reporter Gene	Cy3

Nucleofection Experiments

1 million HaCaT cells at 90 % confluency were nucleofected with 1.5 µg cyclin D1 or ADAM17 specific siRNA using Kit V, program U-020; knock-down of approx. 75 respectively 90 % was achieved. Cy3-labeled siRNA was used as control and transfection efficiency was determined as ~95 %.

Cell/Experiment Datasets

Cell	HaCaT
Cell Type	Skin/Hair Epith.
Tissue Origin	Dermal
Number of Transfected Cells	1 x 10^6
Transfection Program	U-020
Transfection Efficiency	~95 %
Gene Knockdown	~75 resp. ~95 %

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