amaxa - Part of the Lonza Group
 
 
You are not logged in. Log in

Citation Details

Nucleofection is a highly effective gene transfer technique for human melanoma cell lines


Authors >Han SY, >Gai W, >Yancovitz M, >Osman I, >Di Como CJ, >Polsky D
In >Exp Dermatol (2008) 17(5): 405-11
Cells used in publication SK-MEL 19
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 29
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 85
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 94
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 100
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 103
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 147
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 173
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 187
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 192
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 197
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 23
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
SK-MEL 31
   Epithelial Cells
   Cell Lines
   Species: human
   Tissue Origin: dermal
Substrate plasmid (general)
siRNA
Substrate Description pRSV-lacZ plasmid, p53 specific siRNA, pmaxGFP, hdm2luc01 firefly luciferase plasmid + pRL-TK
Applications >RNAi
>Co-Transfection
Topics >Comparison to other transfection methods
>Luciferase
>Microscopy of nucleofected cells
Research Area >Dermatology/Tissue Engineering
Relevant Products > Nucleofector Device
> NHEM-Neo (Normal Human Epidermal Melanocyte-Neonatal) Nucleofector Kit
> Cell Line Nucleofector Kit R
> Cell Line Nucleofector Kit T
> Cell Line Nucleofector Kit V
Reporter Genes maxGFP
beta-Gal
Renilla Luciferase
Firefly Luciferase
Vector Backbones >pmaxGFP
>pRL-TK
>pRSV

Cell/Experiment Datasets

Cell SK-MEL 19
Cell Type Skin/Hair Epith.
Number of Transfected Cells 2x10^6
Transfection Program U-020
Transfection Efficiency 70-80 %
Viability 50-60 %
Determination Method microscopy


> Open PubMed page for this citation

> Back to Citation List

    

What’s Important to You?

Tell us how you like to do your transfection experiments & enable us to serve you better!