Citation Details

Thiopental protects human T lymphocytes from apoptosis in vitro via the expression of heat shock protein 70

Authors	Roesslein M, Schibilsky D, Muller L, Goebel U, Schwer C, Humar M, Schmidt R, Geiger KK, Pahl HL, Pannen BH, Loop T
In	J Pharmacol Exp Ther (2008) 325(1): 217-25
Genes of Interest	hspA1A
Cells used in publication	Jurkat (DSMZ) Blood/Immune Cells Cell Lines Species: human Tissue Origin: blood
Related cells	Jurkat (ATCC) Jurkat Jurkat (ECACC)
Substrate	siRNA
Application	RNAi
Topic	Apoptosis
Research Area	Immunology / Hematology
Relevant Products	Nucleofector Device Cell Line Nucleofector Kit V
Reporter Gene	Alexa

Nucleofection Experiments

Jurkat T cells in logarithmic growth phase were washed in phosphate buffered saline. 2.5 x 10^6 - 5 x 10^6 cells were transfected with 6 μg hspA1A siRNA directed against hsp70-mRNA or nonsilencing siRNA. Transfection efficiency was determined by Alexa fluor 488 labelled hspA1A-siRNA and was > 90%.

Cell/Experiment Datasets

Cell	Jurkat (DSMZ)
Cell Type	T Cell
Tissue Development Stage	Adult
Tissue Origin	Blood
Number of Transfected Cells	2.5-5 x 10^6
Transfection Efficiency	>90 %

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