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Citation Details

Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms


Authors >Nakayama H, >Yoshizaki F, >Prinetti A, >Sonnino S, >Mauri L, >Takamori K, >Ogawa H, >Iwabuchi K
In >J Leukoc Biol (2008) 83(3): 728-41
Genes of Interest Lyn (Src family kinase) signal transduction of neutrophils
Cells used in publication HL-60
   Blood/Immune Cells
   Cell Lines
   Species: human
   Tissue Origin: blood
Substrate siRNA
Substrate Description siRNA for human Lyn (siGenome SMARTpool reagent, Dharmacon) and nontargeting siRNA (control siRNA)
Application >RNAi
Topic >Signal transduction
Research Area >Immunology / Hematology
Relevant Products > Nucleofector Device
> Cell Line Nucleofector Kit V
Reporter Gene siRNA

Cell/Experiment Datasets

Cell HL-60
Cell Type Leukocyte (unspec.)
Number of Transfected Cells 4 x 10^6
Determination Method western blot
Gene Knockdown 67 %


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