Citation Details

Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells

Authors	de la Fuente J, Manzano-Roman R, Blouin EF, Naranjo V, Kocan KM
In	BMC Infect Dis (2007) 7(1): 110
Genes of Interest	Sp110, PSGL-1, ARP-1
Cells used in publication	HL-60 Blood/Immune Cells Cell Lines Species: human Tissue Origin: blood
Related cells	HL-60 (ATCC) HL-60 (DSMZ) HL-60 (ECACC)
Substrate	siRNA
Applications	RNAi 96-well Transfection
Topic	Diseases (e.g. HIV)
Research Area	Immunology / Hematology
Relevant Products	96-well Shuttle Cell Line 96-well Nucleofector Kit SF

Nucleofection Experiments

The expression of Sp110 was silenced in HL-60 cells using combination of two different siRNAs, and to actin-related protein 3 (ARP3) and P-selectin glycoprotein ligand-1 (PSGL-1). After nucleofection cells were divided into two 96-well plates, one for assessment of cell viability and morphology, the second for incubation with Anaplasma phagocytophilum.

Cell/Experiment Datasets

Cell	HL-60
Cell Type	Leukocyte (unspec.)
Tissue Development Stage	Adult
Tissue Origin	Blood
Number of Transfected Cells	5x10^5 per well
Transfection Program	96-EN-138
Transfection Efficiency	87+/-13 %
Viability	63+/-1 %
Determination Method	RT-PCR
Gene Knockdown	Sp110 85+/-9 %

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