Citation Details
RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy
| Authors |  Coughlin CM, Vance BA, Grupp SA, Vonderheide RH |
| In | Blood (2004) 103: 2046-2054 |
| Cells used in publication | B cell, human Human B cells Blood/Immune Cells Primary Cells Species: human Tissue Origin: blood Dendritic cell, human Human dendritic cells (DC) Blood/Immune Cells Primary Cells Species: human Tissue Origin: blood |
| Substrate | mRNA |
| Topics | Differentiation of nucleofected cellsDiseases (e.g. HIV)Intercellular interaction/communicationRNA transfection |
| Research Area | Immunology / Hematology |
| Relevant Products |  Nucleofector Device Human B Cell Nucleofector Kit Human Dendritic Cell Nucleofector Kit I (for short-term expression) |
Research Field
Regarding cancer therapy using vaccination with antigen-presenting cells (APCs), CD40-activated B cells transfected with RNA may serve as an alternative vaccine that can be generated from small blood volumes, allowing the use of this technique also in children.
Nucleofection Experiments
Primary dendritic cells (DCs) and CD40-activated B cells were nucleofected with mRNA coding for GFP, the influenza-derived epitope FluMP, or the tumor rejection antigen MART-1, respectively.
In addition, CD40-activated B cells were transfected with pooled mRNA from 3 neuroblastoma cell lines or with RNA prepared from autologous tumor.
All APCs were able to induce cytotoxic T lymphocytes (CTLs) that secreted interferon-gamma and killed targets.
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