Citation Details

Caspase-3 has a nonapoptotic function in erythroid maturation

Authors	Carlile GW, Smith DH, Wiedmann M.
In	Blood (2004) 103(11): 4310-4316
Genes of Interest	Caspase-3
Cells used in publication	CD34+ cell, human    Human CD34+ hematopoietic progenitor cells    Stem Cells    Primary Cells    Species: human    Tissue Origin: blood
Substrate	Plasmid (general) siRNA
Applications	RNAi Co-Transfection
Topics	Apoptosis Differentiation of nucleofected cells Microscopy of nucleofected cells Signal transduction
Research Area	Immunology / Hematology
Relevant Products	Human CD34 Cell Nucleofector Kit
Reporter Gene	GFP unspecified
Vector Backbone	pEGFP

Research Field

Caspase-3 is an effector caspase cleaving target proteins in the apoptosis signaling cascade. In addition, the authors demonstrate that – independently from apoptosis – it is also required for efficient erythropoiesis.

Nucleofection Experiments

Primary CD34+ erythroblast cells derived from human peripheral blood were co-nucleofected with a plasmid expressing GFP and an siRNA construct targeted against caspase-3. Down-regulation of caspase-3 resulted in a reduction of cells that underwent enucleation with no change in the fraction of cells undergoing apoptosis.

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