Citation Details

Small Interfering RNA-Mediated Knockdown of Notch Ligands in Primary CD4+ T Cells and Dendritic Cells Enhances Cytokine Production

Authors	Stallwood Y, Briend E, Ray KM, Ward GA, Smith BJ, Nye E, Champion BR, McKenzie GJ
In	J Immunol (2006) 177(2): 885-95
Cells used in publication	T cell, unstim., human Human unstimulated T cells Blood/Immune Cells Primary Cells Species: human Tissue Origin: blood Dendritic cell, human Human dendritic cells (DC) Blood/Immune Cells Primary Cells Species: human Tissue Origin: blood
Related cells	T cell, stim., human
Substrate	Plasmid (general) siRNA
Applications	RNAi Co-Transfection
Topics	Differentiation of nucleofected cells Intercellular interaction/communication Luciferase Signal transduction
Research Area	Immunology / Hematology
Relevant Products	Nucleofector Device Human Dendritic Cell Nucleofector Kit I (for short-term expression) Human T Cell Nucleofector Kit
Reporter Genes	Renilla Luciferase Firefly Luciferase
Vector Backbones	pRL-TK pCMV