Citation Details

LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex

Authors	Mi S, Lee X, Shao Z, Thill G, Ji B, Relton J, Levesque M, Allaire N, Perrin S, Sands B, Crowell T, Cate RL, McCoy JM, Pepinsky RB
In	Nat Neurosci (2004) 7(3): 221-8
Cells used in publication	Neuron, hippo/cortical, rat    Rat hippocampal/cortical neurons    Neural/Glial Cells    Primary Cells    Species: rat    Tissue Origin: brain
Substrate	Plasmid (general)
Topics	Cell morphology/cytoskeleton Expression of dominant-negative protein Intercellular interaction/communication Microscopy of nucleofected cells
Research Area	Neurobiology
Reporter Gene	GFP unspecified

Research Field

Axon regeneration in the adult CNS is prevented by inhibitors in myelin that bind to the Nogo-66 receptor / p75 signaling complex and lead to activation of the small GTPase RhoA. LINGO-1, a nervous system-specific transmembrane protein, is a component of the signaling complex.Axon regeneration in the adult CNS is prevented by inhibitors in myelin that bind to the Nogo-66 receptor / p75 signaling complex and lead to activation of the small GTPase RhoA. LINGO-1, a nervous system-specific transmembrane protein, is a component of the signaling complex.

Nucleofection Experiments

Primary P7 rat culture cerebellar granular neurons were nucleofected with the dominant negative form DN-LINGO-1, lacking the cytoplasmic domain involved in binding the Nogo-66 receptor /p75 signaling complex.

DN-LINGO-1 transfected neurons built longer axons due to a diminished responsiveness to myelin inhibition. In the absence of myelin, no axon outgrowth could be observed. The cells also showed a decreased level of RhoA activation.

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