FAQ Database - Complete List

205 FAQ articles available.

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1. What is the basic principle of the Nucleofector® technology?

2. Why is the Nucleofector® technology ideal for primary cells and difficult-to-transfect cell lines?

3. What optimization is necessary to get the technology to work?

4. What can I do when my cell of interest is not in your range of products?

5. What is the best way to establish the Nucleofector® technology in my lab?

6. amaxa suggests using 4-5 million neural cells per 100µl nucleofection® reaction. Why is such a high cell number required? How can I perform nucleofections with fewer cells ?

7. I'm transfecting neurons in a mixed glial culture. Is there an 'optimal' way to influence cell survival and select neurons after nucleofection®?

8. Is there a special recommendation on Human T cell enrichment before nucleofection®?

9. Are there any recommendations to avoid excessive mortality during T cell nucleofection®?

10. How can I increase cell viability of Dendritic cells after nucleofection®?

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