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  1. Do you have any siRNA nucleofection results using concentrations lower than 50nM?

  2. On average how big are hepatocytes?

  3. How many hepatocytes can be obtained from one rat liver?

  4. How many hepatocytes can be obtained from one mouse liver?

  5. The animal facility which isolates my hepatocytes is at a different location, should I be concerned about transport time?

  6. How much DNA can I use when transfecting hepatocytes with the standard Nucleofector®?

  7. For hepatocytes, is plating density a concern post nucleofection®?

  8. Four hours after nucleofection®, I can see the hepatocytes have attached to the well, but the morphology does not look correct, should I be concerned?

  9. As hepatocytes are a very large cell type, should I be worried about nucleofection® causing cell fusions?

  10. What is the average yield of hepatocytes post nucleofection®?

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