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195 FAQ articles available.

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  1. Can I use larger cuvettes? Can I use the cuvettes more than once?

  2. If I use Miltenyi paramagnetic beads for positive selection of cells, i.e. beads remain on the cell surface, does that influence nucleofection results?

  3. What is the proof that DNA enters the nucleus?

  4. How can I remove the cells from the cuvette? Is there any alternative?

  5. What method can I use to fix my HL-60 cells as shown in your optimized protocol?

  6. Do you have a kit for murine embryonic stem cells (mES)?

  7. I routinely culture neural stem cells as neurospheres. Can these be transfected by nucleofection®?

  8. Is your pmaxGFP control vector suitable for stable expression?

  9. Do you have any recommended methods for detaching human monocytes from the plate for assaying?

  10. How do you recommend that we isolate splenic lymphocytes from mouse spleens? Is erythrocyte lysis required?

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