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  1. I am currently working with HUVEC cells. Do you have any recommendations for increased viability post nucleofection®?

  2. Do NSCs (Neural Stem Cells) differentiate in co-culture with astrocytes?

  3. Does your Nucleofector Solution contain anything that would inhibit attachment of adherent cells?

  4. What are your recommendations for minimum and maximum cell numbers for Nucleofection?

  5. Does your system work for cotransfections of plasmids?

  6. How do you determine cell death?

  7. What is the optimal size of DNA that you recommend for Nucleofection?

  8. How should I purify my DNA for Nucleofection of neurons?

  9. I've noticed that duration of the program seems to change even when I haven't changed the program I am using. Why?

  10. What is the lowest amount of DNA which can be used for transfection? Do I need a DNA carrier for low DNA amounts?

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