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195 FAQ articles available.

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31. Do you recommend a particular supplier for siRNA duplexes?

32. Can I use the protocol for MCF7 cells for other breast cancer cell lines?

33. When nucleofecting cancer cell lines, do I need to be concerned about passage number?

34. Why am I not able to detect my fluorescent label (e.g. FITC) after 24 or 48 hours post-nucleofection?

35. Where can I buy media for keratinocytes?

36. For keratinocytes, the note in your optimized protocol says that "primary keratinocytes cultured on feeder-layers MAY need a completely different protocol." What does this refer to?

37. I have been having trouble with "clumping" with my PC12 cells. Do you have any recommendations?

38. Are clonal variations a concern when working with Jurkat cells?

39. What is a Neural Stem Cell (NSC)?

40. Do I need a pure neuronal culture for Nucleofection?

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