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  1. I currently work with some adherent cell lines, such as 293, which are grown in DMEM media. I have noticed that the mortality seems to be high after Nucleofection. Do you have any recommendations?

  2. I just got an "Error 8" message on my Nucleofector. What does this mean?

  3. Can I use the Nucleofector technology for RNAi applications? How do I start?

  4. Can I order any of the kit components separately?

  5. Do you have any helpful calculations or conversion information for siRNA?

  6. Who is the distributor for my country?

  7. Why do you have different Optimized Protocols for unstimulated and stimulated Human T cells?

  8. Is it possible to use frozen primary CD34+ hematopoietic stem cells for nucleofection?

  9. Is it possible to transfect mRNA of tumor cells into antigen-presenting cells like B cells or dendritic cells with the Nucleofector Technology?

  10. What are the critical steps for successful transfection of monocytic cell lines like THP-1, HL60 and U937?

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