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  1. I want to stimulate Human T cells post nucleofection. Are there any precautions to keep in mind?

  2. Can Normocin™, Primocin™, or Plasmocin™ be used as a selection agent?

  3. Is Normocin™ compatible for use in Tetracycline inducible systems?

  4. Is it possible to get neomycin resistant clones after Nucleofection with pmaxGFP?

  5. Where can I find the expiration dates for the kit and the Nucleofector solution?

  6. Can I use the Nucleofector technology for cell fusion applications?

  7. Do you still sell the "prevention" size package of Plasmocin™?

  8. What is the shelf-life of the Nucleofector kits?

  9. My Nucleofector solution was frozen. Is this a problem?

  10. I have a GFP construct that I use and usually get anywhere from 50-80% GFP positive cells using FACS analysis while I get only 30-40% using a GFP-NFAT fusion protein driven by the same promoter. Do you have any suggestions?

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