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195 FAQ articles available.

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  1. What should I pay attention to when transferring my reporter gene set up from a cell line to a primary cell?

  2. What are the best time points for analysis of my siRNA experiments?

  3. Can I co-transfect oligonucleotides and plasmids with amaxa’s technology?

  4. Why does amaxa recommend using non-ventilated caps when culturing insect cells?

  5. What is the ratio of Supplement to Nucleofector® solution?

  6. What are useful methods to enrich/purify specific hematopoetic cell populations before nucleofection®?

  7. Is there an advantage of the MACS® Selection Kit (Miltenyi Biotec GmbH) compared to the RosetteSep® Kit (StemCell Technologies Inc)?

  8. Do you recommend a specific purification method for the selected hematopoetic cell population before nucleofection®?

  9. MACS® Selection Kits (Miltenyi Biotec GmbH) offer the opportunity to purify specific blood/immune cell populations by positive selection via antibody-binding or by negative selection using depletion. Which MACS purification method is preferred by amaxa?

  10. amaxa recommends using PEG purified DNA for nucleofection® of DC's and macrophages. Is it necessary to perform the PEG purification step for the positive control plasmid pmaxGFP® supplied in each kit?

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