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214 FAQ articles available.

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  1. Should I transfect circular or linear plasmid DNA to obtain stable transfectants?

  2. A positive control plasmid works, but I cannot obtain stable clones with my construct of interest. What could be the reason?

  3. How long does it take to obtain stable transfectants?

  4. I have a very high transfection efficiency but most of my cells die during selection, is this to be expected?

  5. What selection markers can I use for stable transfections?

  6. When should I start selection of stable integration?

  7. How often should I change the medium during selection of stables?

  8. How many stable clones will I get from one transfection?

  9. I cannot obtain stable clones from single cells, what could be the reason?

  10. What antibiotic concentration should I use for selecting stable transfectants?

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