Details for amaxa FAQ article #9

«Is there a special recommendation on Human T cell enrichment before nucleofection®?»

For Nucleofection it is preferable to use Human T cell populations
enriched by magnetic separation. For example, MACS® Microbeads (Miltenyi) or by a rosetting method.

For magnetic separation we recommend negative selection or detaching beads after enrichment. Do not apply protocols using hypo-osmolar buffers.
This may lead to high cell mortality after nucleofection.