Details for amaxa FAQ article #6

«What is the best way to establish the Nucleofector® technology in my lab?»

We strongly recommend establishing the Nucleofector technology with the positive control vector pmaxGFP® provided in our kits. pmaxGFP encodes the green fluorescent protein (GFP) from Copepod Pontellina sp. Just like eGFP expressing cells, maxGFP® expressing cells can easily be analyzed by fluorescence microscopy or flow cytometry to monitor transfection efficiency.