Details for amaxa FAQ article #48

«What are your recommendations for minimum and maximum cell numbers for Nucleofection?»

The recommended cell number will vary depending on which Optimized protocol is being used. In general, using less than 2x10⁵cells per reaction causes a major increase in cell mortality. For some cell lines we have tried cell numbers up to 3x10⁷. The problem here is that you end up with a very large cell pellet leading to a dilution of the nucleofector solution as the maximum cuvette volume is 100µL to 110µl.
We suggest increasing the cell number successively from experiment to experiment but not exceeding a cell pellet volume of 50 µl.