Details for amaxa FAQ article #345

«What are the appropriate culture systems for protein production experiments?»

Suspension culture is the predominant culture format for protein production in mammalian cells. For each culture volume, specific culture systems and shaking speeds are required. We recommend the following culture formats and shaking speeds for the different incubation volumes:

25-50 ml culture 125 ml disposable Erlenmeyer flask (e.g. Corning, #431405), shaking at approx. 150 rpm

50-100 ml culture 250 ml disposable Erlenmeyer flask (e.g. Corning, #431407), shaking at approx. 135 rpm

100-200 ml culture 500 ml disposable Erlenmeyer flask (e.g. Corning, #431401), shaking at approx. 125 rpm

200-400 ml culture 1 Liter disposable Erlenmeyer flask (e.g. Corning, #431403), shaking at approx. 110 rpm

1 Liter to 10 Liter culture Spinner flask, stirrer tank, or bioreactor (e.g. Wave™ bioreactors, GE Healthcare)

For volumes above 25 ml, the speed of the orbital shaker may be reduced from 150 rpm to 110-135 rpm in order to prevent foaming. For volumes higher than 1 Liter, the use of spinner flasks, stirrer tanks or bioreactors (e.g. Wave™ bioreactors , GE Healthcare) is recommended in order to ensure sufficient aeration.