Details for amaxa FAQ article #336

«Can I use nucleofection to create a stable knockdown in a cancer cell line?»

Yes. There are several publications available showing stable transfection of an shRNA vector:
Cai S et al. (2006) Nat Genet 38:1278-88 (D10.G4.1 cells)
Guo F et al. (2005) Cancer Res 65:10536-44 (K562)
Hideshima T (2006) Blood 107: 4053-62 (U937)
Ng (2006) Cancer Res 66(20): 9903-12

For generation of stable cell lines, please refer to our amaxa technote by clicking on the associated file "Stable Transfection Guidelines".