Details for amaxa FAQ article #333

«amaxa recommends using PEG purified DNA for nucleofection® of DC's and macrophages. Is it necessary to perform the PEG purification step for the positive control plasmid pmaxGFP® supplied in each kit?»

No, it is not. The PEG purification step removes additional endotoxin from the DNA preparation. The pmaxGFP reporter plasmid supplied in each kit is highly purified and endotoxin levels are <0.01E.U./µg DNA (which means <1pg endotoxin/µg DNA).