Details for amaxa FAQ article #332

«MACS® Selection Kits (Miltenyi Biotec GmbH) offer the opportunity to purify specific blood/immune cell populations by positive selection via antibody-binding or by negative selection using depletion. Which MACS purification method is preferred by amaxa?»

We recommend using negative selection (depletion) because your purified cells are “untouched”, not influenced, and strictly not activated.
Positive selection may cause increasing amounts of dead cells and could also lead to activation of the cells due to binding to a specific ligand.
Please click on the file "Hematopoetic Cell Purification Method.pdf" to see the methods that were tested by amaxa.