Details for amaxa FAQ article #320

«Why does amaxa recommend using non-ventilated caps when culturing insect cells?»

The reason is that insect cells are often incubated in very simple incubators that are not humidified (and without CO₂ at 24°C to 28°C). Insect media typically do not need CO₂ for buffering pH, so gas exchange is not required. To avoid evaporation of medium in a simple incubator that is not humidified, non-ventilated caps have to be used. If a conventional cell culture incubator with humidification is used, ventilated caps can be used as well.