Details for amaxa FAQ article #271

«What antibodies can be used against the GFP produced by pmaxGFP® and pmaxFP®-Green vectors? What antibodies can be used to detect denatured GFP? What antibodies can be used to detect the RFP expressed by pmaxFP-Red?»

Most of the commercial antibodies for GFP do not detect GFP expressed from pmaxGFP or pmaxFP-Green vectors. The origin of the protein expressed by amaxa's pmaxGFP control plasmid and pmaxFP-Green is from the green fluorescent protein CopGFP, cloned from copepoda Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda).
Most commercial antibodies for GFP are designed to react with proteins isolated from other organisms. For example, Clontech`s GFP is from Aequorea victoria (jellyfish), and would not react with antibodies to pmaxGFP or pmaxFP-Green.

Evrogen offers antibodies against the copepod GFP which can be used to detect the GFP expressed by our vectors:
>Anti-GFP, Cat. No. AB501 and AB502 are used against non-denatured protein produced by pmaxGFP
>Anti-TurboGFP antibodies Cat. No. AB511 and AB512 are used against non-denatured protein expressed from pmaxFP-Green
>Anti-TurboGFP(d) antibodies Cat. No. AB513 and AB514 will recognize denatured protein expressed from both pmaxGFP and pmaxFP-Green vectors.

The pmaxFP-Red is identical to JRed from Evrogen. For detection of the RFP expressed by amaxa's pmaxFP-Red, the Anti-KillerRed antibody from Evrogen,
catalog numbers AB961 and AB962, will need to be used.

For more information about these antibodies, please visit Evrogen's website under related links.