Details for amaxa FAQ article #234

«Do you have data about the in vivo implantation of hepatocytes transfected by nucleofection®? For how long could protein expression be detected?»

There is a publication reporting on the successful reinjection of modified hepatocytes into mice. Chen et al. were able to monitor the expression over several days. This group worked with a preliminary test solution for mouse hepatocyte as the optimized protocol was not finished at this time.

Here are the citation details:
Plasmid-electroporated primary hepatocytes acquire quasi-physiological secretion of human insulin and restore euglycemia in diabetic mice
Authors: Chen NK, Sivalingam J, Tan SY and Kon OL
In: Gene Ther (2005) 12(8): 655-667