Details for amaxa FAQ article #21

«My Nucleofector solution was frozen. Is this a problem?»

As long as the supplement was not added to the Nucleofector solution, then there is no risk of any damage to the solutions. Even long-term storage of several months did not alter the nucleofector solutions.
However, if the supplement was added and then frozen, check to see if there is any precipitate. If there is not, then the solution should be fine. If precipitate is present, it may be worthwhile to do a test Nucleofection to make sure the solution is working properly.