Details for amaxa FAQ article #199

«Do I need a special incubator for cultivation of insect cells ?»

Insect cells are cultivated at lower temperatures than E.coli or mammalian cells (S2 cells at 24°C, Sf9 cells at 28°C). Therefore they need a dedicated incubator with appropriate temperature. Since insect media typically do not need CO2 and evaporation can be avoided by closing the cap of the culture flask simple incubators are sufficient