Details for amaxa FAQ article #179

«Do you have a protocol for the nucleofection® of Plasmodium yoelii?»

Yes. You can use our new Basic Parasite Nucleofector® Starter kit for parasitic protozoa. The Basic Parasite Nucleofector Starter Kit (Cat.No. VMI-1001) should help you to determine the optimal program and Nucleofector Solution for your parasite-of-interest before using either
Basic Parasite Nucleofector Kit 1 or 2.
We have excellent data for Plasmodium yoelii published in Molecular And Biochemical Parasitology. Details of the nucleofection procedure are described in: Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii. Authors: Jongco AM, Ting LM, Thathy V, Mota MM, Kim K. In: Mol Biochem Parasitol (2006) 146(2): 242-250