Details for amaxa FAQ article #160

«What kind of protocol you recommend for stimulation of human T cells?»

For getting the results we present in our datasheet, we recommend the follwing strategy:
Cells should be stimulated and cultivated for 3 days before nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody. Alternatively, cultivate with 2 µg/ml PHA for 24 hours, wash cells once with medium the following day and add fresh medium supplemented with 25 U/ml IL-2 .
Replace medium every 2-3 days and cultivate the cells for 3 days before nucleofection to get high transfection efficiencies.

To see some representative data, please click on the associated PDF file.