Details for amaxa FAQ article #140

«Does the Mouse T cell protocol work for stimulated mouse T cells?»

Yes. Stimulate the cells with anti CD3 and anti CD28 antibody for 24 hours. Use the optimized protocol and program X-001. Often times, this will result in higher transfection efficiency (60-70% is possible).

Alternatively, you can stimulate with ConA and IL-2 for 2-3 days.