Details for amaxa FAQ article #134

«What kind of suspension CHO cells did you use for protein production?»

The data we show in our application note for protein production was generated with 2 different suspension CHO clones. This protocol tested for the suspension CHO clones provided by ECACC [Cat. No. 102307] and from Invitrogen, CHO-S Cells, SFM Adapted [Cat no # 11619-012].

Please use the recommended medium for the Invitrogen cells:
CD CHO Medium (Invitrogen; Cat. No. 10743) with 10 ml/l HT Supplement (Invitrogen; Cat. No. 11067) and 8 mM L-Glutamine

Relations for this FAQ article

Research Areas	Cancer Research/Cell Biology Dermatology/Tissue Engineering
Categories	General Product Information General Protocol Information Cell Prep. And Handling Cell Culture Reagents
Cells	CHO [suspension] (ECACC) CHO-S [suspension] (Invitrogen)
Products	VCA-1003 - Cell Line Nucleofector Kit V VGA-1003 - cGMP Cell Line Nucleofector Kit V
Applications	Protein Production
Related links	http://www.amaxa.com/transientproteinprod.html http://www.amaxa.com/cho-sus.html http://www.ecacc.org.uk/
Files	Protein_Production_Flyer.pdf

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