Details for amaxa FAQ article #122

«Four hours after nucleofection®, I can see the hepatocytes have attached to the well, but the morphology does not look correct, should I be concerned?»

There is no reason for alarm. The hepatocytes may not exhibit normal morphology a few hours after nucleofection but by 24 hours post nucleofection, morphology should be normal. Remember to perform a fluid change about 4 hours post nucleofection.