Details for amaxa FAQ article #10

«Are there any recommendations to avoid excessive mortality during T cell nucleofection®?»

Try to keep the DNA amount for nucleofection quite low (e.g. 1 µg plasmid DNA) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. Endofree® Plasmid Kits (Qiagen).

The A260:A280 ratio should be at least 1.8 for nucleofection.
Please click on the related link for more information about Qiagen's purification kits.