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FAQ Database - Complete List

195 FAQ articles available.

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  1. Do you recommend positive selection or depletion for the purification of Human monocytes?

  2. Does it matter if the PBS used for monocyte enrichment contains calcium and magnesium?

  3. What is the sequence of the siRNA oligo supplied in your siRNA Test Kit?

  4. How can I determine the correct centrifugation speed for my particular centrifuge and rotor?

  5. Is the age of the mice important for my mouse T cell nucleofection?

  6. Does the recovery medium contained in the Mouse T Cell and Human Monocyte Nucleofector® Kits contain any immunogenic substances?

  7. What is the maximum insert size that can be cloned into your pmaxFP® vectors?

  8. I see activation of my monocytes (or macrophages or DCs) following nucleofection. Why is this? What can I do to address this problem?

  9. I observed high mortality rates in mES cells after Nucleofection with a Cre recombinase expression vector. How can I increase the viability?

  10. Why do your new pmaxFP® vectors contain an SV40 ori sequence?

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