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FAQ Database - Complete List

195 FAQ articles available.

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  1. Can low A260:A280 ratios lead to both reduced transfection efficiency and cell viability?

  2. Can nicked DNA lead to reduced transfection efficiency?

  3. Can I use nucleofection to create a stable knockdown in a cancer cell line?

  4. What protocol should I use for nucleofection® of patient derived blood samples, e.g. leukemia or lymphoma cells?

  5. Is it possible to transfect siRNA into blood cells from NHL patients?

  6. I have great efficiency for my cancer cell line with pmax-GFP but lower results with my construct. Do you have any suggestions on how to improve the efficiency?

  7. When working with parasites, if I was previously using the test kit with the 88A6 solution with satisfactory results, which kit should I use now?

  8. When working with parasites, if I was previously using the human T cell kit, what kit should I use?

  9. My suspension-adapted HEK293 (or CHO) cells tend to clump in culture. How can I avoid this?

  10. What are the appropriate culture systems for protein production experiments?

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