Nucleofection of K-562
| Cat. No. | VCA-1003 |
| Optimized Protocol |  Download (PDF, 131 KB) |
| Transfection Efficiency | After 5 hours: 82±2% After 24 hours: 89±3% |
| Cell Database |  K-562 (ATCC) |
| Cell Clone |  ATCC CCL-243 |
| Cat. No. | VCA-1003 |
| Optimized Protocol | Download (PDF, 284 KB) |
| Transfection Efficiency | After 5 hours: 69±7% After 24 hours: 79±5% |
| Cell Database | K-562 (DSMZ) |
Nucleofection of K-562 (ATCC) cells.
K-562 (ATCC) cells were nucleofected using the Cell Line Nucleofector Kit V, program T-16 and 2.5 µg of a plasmid encoding the enhanced green fluorescent protein eGFP.
5 hours post nucleofection, the cells were analyzed by light (top) and fluorescence microscopy (bottom).
The human chronic myeloid leukemia derived cell line K-562 is often used as a model system to address scientific problems of hematological relevance. Using the Optimized Nucleofector Protocol K-562 cells can be easily and reproducibly transfected with high efficiencies. Due to the immediate nuclear transport of the DNA, the expression of a transfected gene can already be detected after a very short incubation period. Efficiencies of up to 50%, for example, can be detected after just 2-4 hours of incubation. Between 1-2x106 cells and 1 to 5 µg of DNA should be used per nucleofection experiment.
Gene transfer efficiencies of K-562 (DSMZ)cells.
K-562 (DSMZ) cells were nucleofected with a plasmid encoding the mouse MHC class I heavy chain molecule H-2Kk. The diagram shows gene transfer efficiencies, given as % transfected of living cells, after 4, 24, 48 and 72 hours.
Components of Cell Line Nucleofector Kit V
Cat. No. VCA-1003, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 30 | µg | pmaxGFP (for 15 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Cell Line Nucleofector Solution V |
