Human CD34⁺ Nucleofector Kit

Cat. No.	VPA-1003
Optimized Protocol	Download (PDF, 248 KB)
Transfection Efficiency	After 20 hours: 71±11%
Cell Database	CD34+ cell, human

In their role as hematopoietic progenitors CD34⁺ cells are of great interest to immunologists. Recently, they have also been shown to differentiate into other non-hematopoietic cells, such as endothelial precursors. So far, transfection of CD34⁺ progenitor cells has only been possible with viral methods or by electroporating stimulated cells. Using nucleofection, unstimulated CD34⁺ cells can now be transfected non-virally. For Nucleofection CD34⁺ cells can be derived from cord blood or leukapheresis material. Both fresh or cryopreserved material can be used.

Up to 70% transfection efficiency
Cited for DNA and siRNA transfection
For unstimulated primary CD34⁺ cells

Example for nucleofection of CD34⁺ hematopoietic progenitor cells with H-2K^k cDNA.

Unstimulated peripheral blood mononuclear cells, partially enriched for CD34⁺ cells were transfected using the Human CD34 Cell Nucleofector Kit and a plasmid encoding the mouse MHC class I heavy chain molecule H-2K^k. 16 hours post nucleofection the cells were stained for CD34 and H-2K^k and analyzed by flow cytometry. Lymphocytes were gated according to forward/side scatter (A). Dead cells and CD34^- cells were excluded by propidium iodide staining and gating (B and C). H-2K^k expression is shown after nucleofection without (D) and with plasmid DNA (E).

Components of Human CD34 Cell Nucleofector Kit

Cat. No. VPA-1003, 25 Reactions

25		Plastic pipettes
25		Certified cuvettes
10	µg	pmaxGFP (for 5 reactions)
0.5	ml	Supplement
2.25	ml	Human CD34 Nucleofector Solution

Human CD34+ Nucleofector Kit

Human CD34⁺ Nucleofector Kit