Human B Cell Nucleofector Kit
| Cat. No. | VPA-1001 |
| Optimized Protocol |  Download (PDF, 203 KB) |
| Transfection Efficiency | After 20 hours: 36±11% |
| Cell Database |  B cell, human |
Efficient transfection of human B cells is a pre-requisite to enable research in fields such as chemokine/antigen response or B cell activation. Now cited in various publications, the Human B Cell Nucleofector Kit has been used for both DNA and RNA transfection and has proven its applicability in many research areas.
- For unstimulated and stimulated B cells
- Up to 40% transfection efficiency
- One protocol for DNA, siRNA, RNA
Example for nucleofection of CD19+ B cells with H-2Kk cDNA.
Fresh, unstimulated PBMC were transfected using Human B Cell Nucleofector Kit and a plasmid encoding the mouse MHC class I heavy chain molecule H-2Kk. 16 hours post nucleofection, the cells were stained for CD19 and H-2Kk. Lymphocytes were gated according to forward/side scatter (A). Dead cells and CD19- cells were excluded by staining with propidium iodide and gating (B, C). H-2Kk expression is shown post nucleofection without (D) and with plasmid DNA (E).
Components of Human B Cell Nucleofector Kit
Cat. No. VPA-1001, 25 Reactions
| 25 | Plastic pipettes | |
| 25 | Certified cuvettes | |
| 10 | µg | pmaxGFP (for 5 reactions) |
| 0.5 | ml | Supplement |
| 2.25 | ml | Human B Cell Nucleofector Solution |
