Details for amaxa FAQ article #31

«Is it possible to transfect mRNA of tumor cells into antigen-presenting cells like B cells or dendritic cells with the Nucleofector Technology?»

Yes. Recently Coughlin and co-workers showed that RNA transfected CD40-activated B cells or DCs induce functional T cell
responses against viral and tumor antigen targets Blood (2004) 103:2046-2054).

In short, the authors found:
>mRNA uptake in B cells and dendritic cells up to 80%
>CD40-activated B cells or dendritic cells induce functional T cell responses against viral and tumor antigen targets.
>mRNA transfection is less toxic and gave higher efficiencies
>Shown by Van de Parre with macrophage cell lines