Citation Details

Efficient nonviral gene delivery into primary lymphocytes from rats and mice

Authors	Goffinet C, Keppler OT
In	FASEB J (2006) 20(3): 500-2
Cells used in publication	T cell, mouse - BALB/c Mouse (BALB/c) unstimulated T cells Blood/Immune Cells Primary Cells Species: mouse Tissue Origin: blood T cell, stim., mouse BALB/c Primary stimulated murine T cell Blood/Immune Cells Primary Cells Species: mouse Tissue Origin: blood T cell, rat Rat T cells Blood/Immune Cells Primary Cells Species: rat Tissue Origin: blood
Substrate	plasmid (general) siRNA
Substrate Description	DNA: pHIV-EGFP fusion, 14kb, expression cassette for HIV Nef protein. siRNA: alexa488 labelled siRNA, hCD4
Application	RNAi
Topics	Comparison to other transfection methods Diseases (e.g. HIV) Gene therapy
Research Areas	Cancer Research/Cell Biology Immunology / Hematology
Relevant Products	Nucleofector Device Human T Cell Nucleofector Kit
Reporter Genes	eGFP Alexa siRNA