Citation Details

Cellular APOBEC3G restricts HIV-1 infection in resting CD4+ T cells

Authors	Chiu YL, Soros VB, Kreisberg JF, Stopak K, Yonemoto W, Greene WC
In	Nature (2005) 435(7038): 108-114
Cells used in publication	T cell, unstim., human    Human unstimulated T cells    Blood/Immune Cells    Primary Cells    Species: human    Tissue Origin: blood
Substrate	siRNA
Application	RNAi
Topic	Diseases (e.g. HIV)
Research Area	Immunology / Hematology
Relevant Products	Nucleofector Device
Reporter Genes	FITC siRNA

Research Field

The anti-retroviral deoxycytidine deaminase APOBEC3G strongly protects unstimulated peripheral blood CD4+ T cells against HIV-1 infection.

Nucleofection Experiments

APOBEC3G-specific siRNAs as well as 2bp-mismatch control siRNAs were introduced into unstimulated CD4+ T cells. Due to the knockdown of APOBEC3G, the early replication block encountered by HIV-1 was greatly relieved.

 Open PubMed page for this citation

 Back to Citation List