Citation Details

Neuropeptide FF (NPFF) analogs functionally antagonize opioid activities in NPFF2 receptor-transfected SH-SY5Y neuroblastoma cells

Authors	Mollereau C, Mazarguil H, Zajac JM, Roumy M
In	Mol Pharmacol (2005) 67(3): 965-975
Cells used in publication	SH-SY5Y    Neural/Glial Cells    Cell Lines    Species: human    Tissue Origin: brain
Related cells	SH-SY5Y (ATCC)
Substrate	Oligopeptides
Topic	Signal transduction
Research Area	Neurobiology
Relevant Products	Nucleofector Device

Research Field

The authors elucidate the mechanisms and receptors involved in the cellular antiopioid activity of neuropeptide FF (NPFF).

Nucleofection Experiments

Using the Nucleofector, peptides corresponding to the C-terminus of several G protein subunits were transferred into the clone SH2-D9, a derivative of the SH-SY5Y neuroblastoma cell line expressing the NPFF2 receptor. Peptide delivery efficiency was 77% as measured with an Alexa-labeled peptide.

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