Citation Details
Efficient gene transfer into murine embryonic stem cells by nucleofection
| Authors |  Lorenz P, Harnack U, Morgenstern R |
| In | Biotechnol Lett (2004) 26(20): 1589-1592 |
| Cells used in publication | Embryonic stem (ES) cell, mouse Mouse embryonic stem (ES) cells Stem Cells Primary Cells Species: mouse Tissue Origin: embryo |
| Substrate | plasmid (general) |
| Application | Stable Transfection |
| Topics | Comparison to other transfection methodsGene therapy |
| Research Area | Cancer Research/Cell Biology |
| Relevant Products |  Mouse ES Cell Nucleofector Kit |
| Reporter Gene | eGFP |
| Vector Backbone | pEGFP |
Research Field
Genetic manipulation of embryonic stem (ES) cells is performed by non-viral as well as viral transfection methods. The authors tested nucleofection for the introduction of a plasmid encoding EGFP into murine ES cells.
Nucleofection Experiments
Transfection effciencies in viable mouse ES cells (murine embryonic stem cell line ES-D3 (ATCC)) were between 85% and 96% with high levels of EGFP expression [mean fluorescence intensity (MFI): 630 +/- 90] 24 h after nucleofection.
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