Citation Details

BAI1 is an engulfment receptor for apoptotic cells upstream of the ELMO/Dock180/Rac module

Authors	Park D, Tosello-Trampont AC, Elliott MR, Lu M, Haney LB, Ma Z, Klibanov AL, Mandell JW, Ravichandran KS
In	Nature (2007) 450(7168): 430-434
Genes of Interest	ELMO1, BAI1
Cells used in publication	J-774    Blood/Immune Cells    Cell Lines    Species: mouse    Tissue Origin: ascites Astrocyte, mouse    Mouse astrocytes    Neural/Glial Cells    Primary Cells    Species: mouse    Tissue Origin: brain
Substrate	plasmid (general) siRNA shRNA plasmid
Applications	RNAi Stable Transfection Co-Transfection
Topics	Apoptosis Intercellular interaction/communication Signal transduction
Research Areas	Cancer Research/Cell Biology Immunology / Hematology
Relevant Products	Nucleofector Device Mouse Astrocyte Nucleofector Kit Cell Line Nucleofector Kit V

Nucleofection Experiments

J-774: stable overexpression of BAI1; stable knockdown of ELMO1 by shRNA construct, co-transfection with BAI-1-GFP construct - apoptosis assays; also transient transfection of BAI1 siRNA (Dharmacon SmartPool), knock-down 20 %.
Murine Astrocytes: Transfection of BAI1 siRNA, knock-down 46 %, resulting in decreased uptake of apoptotic thymocytes.

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