Citation Details

Preferential expression of very late antigen-4 on type 1 CTL cells plays a critical role in trafficking into central nervous system tumors

Authors	Sasaki K, Zhu X, Vasquez C, Nishimura F, Dusak JE, Huang J, Fujita M, Wesa A, Potter DM, Walker PR, Storkus WJ, Okada H
In	Cancer Res (2007) 67(13): 6451-8
Genes of Interest	CD49d (mouse), heterodimer with CD29: homing receptor VLA-4 (very late antigen) for Tc1 (type 1 cytotoxic T lymphocyte)
Cells used in publication	T cell, mouse - C57BL/6    Mouse (C57BL/6) unstimulated T cells    Blood/Immune Cells    Primary Cells    Species: mouse    Tissue Origin: blood
Related cells	T cell, stim., mouse BALB/c T cell, mouse thymus
Substrate	siRNA
Substrate Description	siRNA sequences specific for mouse CD49d #67813[sense, 5-GGAUGUUUGGACAAUCUAUTT-3; antisense, 5-AUAGAUUGUCCAAACAUCCTT- 3] and a nontargeting siRNA #4 (Ambion, Inc.) were synthesized and annealed by the manufacturer (Ambion).
Application	RNAi
Topic	RNA transfection
Research Areas	Cancer Research/Cell Biology Immunology / Hematology Neurobiology
Relevant Products	Nucleofector Device Mouse T Cell Nucleofector Kit
Reporter Gene	siRNA

Nucleofection Experiments

Excellent data from long-term (9 days) stimulated CD8+ cytotoxic T lymphocytes

- in vivo data

- functional data (migration)

- siRNA knockdown of > 50 %

Cell/Experiment Datasets

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