Efficient Delivery and Knockdown by Nucleofection of siRNA Duplexes
As proven by more than 300 publications the Nucleofector Technology enables researchers to perform succesful siRNA experiments in primary cells and difficult-to-transfect cell lines such as suspension cells. Depending on cell type and target, efficient knockdown can be observed at siRNA concentrations lower than 10 nM.
- siRNA delivery with up to 99% efficiency
- Low cytotoxicity
- Co-transfection with plasmid DNA for transfection control or rescue experiments
- siRNA library screening in difficult-to-transfect cell types using the
96-well Shuttle
Nucleofection outperforms lipofection for effective GAPDH mRNA knockdown in difficult-to-transfect cell types.
Cells (all ATCC) were transfected with 5 pmol SMARTpool reagent (Dharmacon) targeting GAPDH using the 96-well Shuttle (according to the respective optimized protocol) or Reagent L (after titration of optimal reagent amount). GAPDH mRNA levels were analyzed 24 hours post-transfection by the QuantiGene branched-DNA assay (Panomics) and normalized to siCONTROL Non-Targeting siRNA #1 (Dharmacon).
Data generated in collaboration with Dharmacon, Inc..
