Accelerate and Standardize Screening of Expression Vector Constructs
96-well nucleofection offers a whole range of new possibilities for protein expression and production by combining:
- Outstanding transfection efficiencies for cell lines most relevant for protein production (e.g. CHO and HEK 293 suspension clones, S2, NS0, SP2/0)
- Unparalleled reproducibility
- Fast procedure
Screening of expression (vector) constructs or generation of baculovirus variants can be performed with unrivaled reproducibility and accuracy.
Workflow of screening of expression vector constructs using 96-well nucleofection.
Reliable well-to-well and lot-to-lot uniformity with the 96-well Shuttle– the ideal pre-requisites for expression vector construct screening
2 x 105 CHO-S cells were transfected with 0.4 µg of an expression vector encoding the SEAP gene using the Cell Line 96-well Nucleofector Kit SG. 24 hours post nucleofection SEAP enzyme activity was measured (n=80, SD = ± 6.1% from mean). Wells without SEAP enzyme activity are negative controls of cells in 96-well Nucleofector Solution and plasmid DNA but without nucleofection.
